PEPVAC

GENOME WIDE PREDICTION OF PROMISCOUS EPITOPES FOR VACCINE DESIGN

T-cell vaccines HLA-peptide binding HLA-coverage Proteosome Cleavage

Function:

PEPVAC is a tool aimed to the development of fully covering multi-epitope vaccines against pathogenic organisms based on genome wide predictions of promiscuous MHCI-restricted epitopes

Description:

T-cell epitopes are first anticipated based on their binding to HLA I molecules using profile-matrices, and then filtered for immunoproteasomal cleavage using a probabilistic model. HLA I molecules present many allelic variants with distinct peptides specificities, and thus peptide binding predictions are given for a set of HLA I alleles with a combined phenotypic frequency ~ 95% in 5 distinct ethnic groups. These HLA I alleles are grouped in sets (supertypes) whose predicted binding peptides are largely overlapping. Only the peptides predicted to bind to all HLA I alleles included in each supertype are returned as potential T-cell epitopes. Identification of these promiscuous peptide binders allows to minimize the total number of predicted epitopes without compromising the population coverage required in the design of multi-epitope vaccines.
E-MAIL
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Enter your e-mail
GENOMES
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Select a genome
Upload genome (File with translated ORFs in FASTA format)
 

SUPERTYPES
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Select HLA-Supertype/s
A2: A*0201, A*0202, A*0203, A*0205, A*0206, A*0207, A6802
A3: A*0301, A*1101, A*3101, A*3301, A*6801, A*6601
A24: A*2402, B*3801
B7: B*0702, B*3501, B*5101, B*5102, B*5301, B*5401
B15: A*0101, B*1501_B62, B1502
Population Coverage with selected supertypes [help]
PROTEASOME CLEAVAGE
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Filter: Model:

Mirror Tool available at Dr. Reche's Immunomedicine Group, Facultad de Medicina, U.C.M., SPAIN.

Hits since June/2004
For questions about this site, please contact Guanglan Zhang
Contact us: Bioinformatics Molecular Immunology Foundation